What to do after appreciating the colony morphology?
Bit of fungal colony——–>LPCB mount——–>view under microscope——–> look for nature of hyphae and type of sporulation
LPCB mount is used to study the microscopic features of fungal isolates grown in culture. It contains phenol which acts as a disinfectant, lactic acid which preserves the morphology of fungi, glycerol which prevents drying and cotton blue which stains the fungal elements blue.
Procedure for slide culture
Take a petri dish——–>Place a bent glass rod on it ——–> On this glass rod place a sterile petri dish——–> place 2 agar blocks of 1cm2 on petri dish ——–>innoculate the margins of agar block with bits of fungal colony——–> Place 2 coverslips on the top of the 2 agar blocks——–>innoculate the Petri dish at 25°C——–> make LPCB mounts——–> viewed under microscope
Take impressions of the colony on the surface of the SDA plate with a cellophane tape
Germ tube test
Sugar fermentation test
Hair perforation test
Immunological methods of identification
Antibody detection by ELISA, CFT, agglutination test, etc
Antigen detection by latex agglutination test
Skin tests to demonstrate the delayed type of hypersensitivity
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